• A SPECTROPHOTOMETRICAL METHOD FOR DETERMINATION OF DISSOLVED PROTEINS IN WATER OR WASTEWATER

    Author(s):
    Aimee E Thayer, Lawrence K Wang (see profile) , Mu-Hao Sung Wang
    Editor(s):
    Hung-ping Tsao, Lawrence K Wang
    Date:
    2019
    Group(s):
    Digital Books, Environmental Humanities, Sustainability, World-Ecology Research Network
    Subject(s):
    Science and technology studies (STS)
    Item Type:
    Book chapter
    Tag(s):
    STEM
    Permanent URL:
    http://dx.doi.org/10.17613/amz9-yz48
    Abstract:
    Wang, LK, Wang MHS and Thayer, AE (2019). A spectrophotometrical method for determination of dissolved proteins in water or wastewater. In: "Evolutionary Progress in Science, Technology, Engineering, Arts, and Mathematics (STEAM)", Wang, Lawrence K. and Tsao, Hung-ping (editors). Volume 1, Number 12, December 2019; 57 pages. Lenox Institute Press, Newtonville, NY, 12128-0405, USA. No. STEAM-VOL1-NUM12-DEC2019; ISBN 978-0-9890870-3-2. --------------- ABSTRACT: The objective of this research are two-fold: (a) to review current methods for rapid analysis of dissolved protein in water and wastewater: and (b) to introduce a new spectrophotometrical method for determination of dissolved proteins in the 10-2500 mg/L range using carbonate-tartrate reagent, phosphomolybdic-phosphotungstic reagent, and copper sulfate. The new analytical method is a colorimetric determination based on the reaction of dissolved protein, specifically the peptide bonds and the amino acids tryptophan and tyrosine with the authors suggested reagents. Two reactions are necessary:(a) The reaction between the protein molecules and copper ions in an alkaline solution; and (b) The reduction of a phosphomolybdic phototungstic reagent by the copper treated protein. The optical density of treated dissolved protein is then determined at 400-700 nm range using a spectrophotometer with 1 cm light path or longer. With certain modification, this method may also be used in the field using the calibration curves prepared in the laboratory.
    Notes:
    KEYWORDS: Literature review, Environmental chemistry, Dissolved protein, Pollutant, Impurity, Water, Wastewater, Glossary, UV-visible spectroscopy, Laboratory waste minimization, Neutralization., STEM, STEAM
    Metadata:
    Published as:
    Book chapter    
    Status:
    Published
    Last Updated:
    11 months ago
    License:
    All Rights Reserved
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