• A Zebrafish Model for Studies on Esophageal Epithelial Biology

    Author(s):
    Hao Chen
    Translator(s):
    james Wilson (see profile)
    Date:
    2015
    Subject(s):
    Public health
    Item Type:
    Article
    Tag(s):
    Zebrafish
    Permanent URL:
    http://dx.doi.org/10.17613/M6D51Z
    Abstract:
    Mammalian esophagus exhibits a remarkable change in epithelial structure during the tran- sition from embryo to adult. However, the molecular mechanisms of esophageal epithelial development are not well understood. Zebrafish (Danio rerio), a common model organism for vertebrate development and gene function, has not previously been characterized as a model system for esophageal epithelial development. In this study, we characterized a piece of non-keratinized stratified squamous epithelium similar to human esophageal epi- thelium in the upper digestive tract of developing zebrafish. Under the microscope, this piece was detectable at 5dpf and became stratified at 7dpf. Expression of esophageal epi- thelial marker genes (Krt5, P63, Sox2 and Pax9) was detected by immunohistochemistry and in situ hybridization. Knockdown of P63, a gene known to be critical for esophageal epi- thelium, disrupted the development of this epithelium. With this model system, we found that Pax9 knockdown resulted in loss or disorganization of the squamous epithelium, as well as down-regulation of the differentiation markers Krt4 and Krt5. In summary, we charac- terized a region of stratified squamous epithelium in the zebrafish upper digestive tract which can be used for functional studies of candidate genes involved in esophageal epithe- lial biology.
    Notes:
    http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0143878
    Metadata:
    Last Updated:
    1 week ago

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