• Moderate Concentrations of TNF- α Induce BMP-2 Expression in Endothelial Cells

    Author(s):
    Oski Illiandri (see profile) , Nur Permatasari, Setyawati Soeharto
    Editor(s):
    Hidajat Sujuti
    Date:
    2016
    Group(s):
    Medical Humanities
    Subject(s):
    History and philosophy of medicine, Literature and medicine, Medicine, Philosophy and medicine
    Item Type:
    Article
    Permanent URL:
    http://dx.doi.org/10.17613/M6F69N
    Abstract:
    Tumor necrosis factor-alpha (TNF-α) is best-known as a potent pro-inflammatory cytokine involved in many cardiovascular diseases. During vascular calcification, TNF-α has been reported topromote osteogenic differentiation of human vascular smooth muscle cells (VSMC) and mesenchymal stem cells (MSCs). In contrast, there is alack of data reporting the osteoinductive effect of TNF-α in endothelial cell. In this study, experiments were performed to investigate and determine theoptimum dose of TNF-α that induces expression of the osteoinductive factor bone morphogenetic protein (BMP-2) in endothelial cells. Human vein endothelial cells were treated with TNF-α at doses of 0, 2, 5, 10, or 20 ng/mL for2, 8, or 24 h time intervals. BMP-2 cell expression was evaluated using immunocytochemistry staining by calculating the percentage of BMP-2 positive cells. Apoptosiswas determined by counting the number ofpyknoticcells. In this study, we found that the optimum dose of TNF-α thatinduces BMP-2 expression in endothelial cells was 5 ng/mL at the 8 h time interval. Lower (2 ng/mL) orhigher (10 and 20 ng/mL) concentrations of TNF-α had minimal effects on BMP-2 expression. Moreover, higher concentrations of TNF-α treatment (10 and 20 ng/ml) at8 h and 24 h increased the presence of pyknotic endothelial cells, which represent thefinal stage of apoptosis.
    Metadata:
    Published as:
    Journal Article
    Journal:
    International Journal of Pharmaceutical and Clinical Research 2016; 8(12): 1666-1669
    Volume:
    8
    Start Page:
    1666
    End Page:
    1669
    Status:
    Published
    Last Updated:
    3 months ago
    License:
    Attribution

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